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Lowenstein Jensen selective media to Reduce contamination in Tuberculosis Culture

  • 2014/07/30
Type de publication
  • Articles
Auteurs
  • Kassaza K
  • Orikiriza P
  • Llosa A
  • Bazira J
  • Nyehangane D
  • Page AL
  • Boum Y
Thèmes
  • Tuberculose
Abstract
We compared Mycobacterium tuberculosis sputum culture recovery and contamination rates between Lowenstein-Jensen medium (LJ) containing the following decontaminants and LJ alone: (i) PANTA (n = 299), (ii) Selectatab-MB (n = 299), and (iii) penicillin G (n = 234). The contamination rate for LJ alone was approximately 31%, versus 5.0% for PANTA-containing, 2% for Selectatab-containing, and 9% for penicillin-containing media (P < 0.001). M. tuberculosis isolation rates were 9.8%, 17%, 18%, and 12% for standard LJ, PANTA, Selectatab, and penicillin cultures, respectively.