Publications

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Field Evaluation of a Simple Fluorescence Method for Detection of Viable Mycobacterium tuberculosis in Sputum Specimens during Treatment Follow-Up

  • 2012/10/17
Type of publication
  • Articles
Authors
  • Schramm B
  • Hewison C
  • Bonte L
  • Jones W
  • Camelique O
  • Ruangweerayut R
  • Swaddiwudhipong W
  • Bonnet M
Themes
  • Tuberculose

Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture.